验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, LNCaP cells, mouse lung tissue, HepG2 cells, K-562 cells, mouse liver tissue, rabbit liver tissue |
| Positive IHC detected in | human thyroid cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
67905-1-Ig targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, rabbit |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag29986 Product name: Recombinant human METTL7A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-244 aa of BC008180 Sequence: MELTIFILRLAIYILTFPLYLLNFLGLWSWICKKWFPYFLVRFTVIYNEQMASKKRELFSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFLIKSIAENRHLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEHVAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALERASFSKLKLQHIQAPLSWELVRPHIYGYAVK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 7A |
| 别名 | AAM B, EC:2.1.1.348, EC:2.1.1.9, methyltransferase like 7A, Methyltransferase-like protein 7A |
| 计算分子量 | 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC008180 |
| 基因名称 | METTL7A |
| Gene ID (NCBI) | 25840 |
| RRID | AB_2918661 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9H8H3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for METTL7A antibody 67905-1-Ig | Download protocol |
| WB protocol for METTL7A antibody 67905-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
