验证数据展示
经过测试的应用
Positive WB detected in | rat liver tissue, LNCaP cells, mouse lung tissue, HepG2 cells, K-562 cells, mouse liver tissue, rabbit liver tissue |
Positive IHC detected in | human thyroid cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 1 publications below |
产品信息
67905-1-Ig targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat, rabbit |
文献引用反应性 | human |
免疫原 | METTL7A fusion protein Ag29986 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | methyltransferase like 7A |
别名 | N6-adenosine-methyltransferase TMT1A, Methyltransferase-like protein 7A, methyltransferase like 7A, EC:2.1.1.9, EC:2.1.1.348 |
计算分子量 | 28 kDa |
观测分子量 | 28 kDa |
GenBank蛋白编号 | BC008180 |
基因名称 | METTL7A |
Gene ID (NCBI) | 25840 |
RRID | AB_2918661 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9H8H3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.
实验方案
Product Specific Protocols | |
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WB protocol for METTL7A antibody 67905-1-Ig | Download protocol |
IHC protocol for METTL7A antibody 67905-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |