• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

METTL7A Monoclonal antibody

METTL7A Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9B4

Cat No : 67905-1-Ig

Print datasheet

Synonyms

AAM B, DKFZp586A0522, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

验证数据展示

  • WB analysis using 67905-1-Ig

    WB analysis using 67905-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67905-1-Ig

    WB analysis using 67905-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67905-1-Ig

    WB analysis of HepG2 using 67905-1-Ig

    WB result of METTL7A antibody (67905-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

  • IHC staining of human thyroid cancer using 67905-1-Ig

    IHC staining of human thyroid cancer using 67905-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL7A antibody included
  • IHC staining of human thyroid cancer using 67905-1-Ig

    IHC staining of human thyroid cancer using 67905-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL7A antibody included

经过测试的应用

Positive WB detected inrat liver tissue, LNCaP cells, mouse lung tissue, HepG2 cells, K-562 cells, mouse liver tissue, rabbit liver tissue
Positive IHC detected inhuman thyroid cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL7A Ready-To-Use IHC Kit. METTL7A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67905-1-Ig targets METTL7A in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, rabbit
Immunogen METTL7A fusion protein Ag29986 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methyltransferase like 7A
Synonyms AAM B, DKFZp586A0522, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC008180
Gene Symbol METTL7A
Gene ID (NCBI) 25840
RRIDAB_2918661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H8H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.

实验方案

Product Specific Protocols
WB protocol for METTL7A antibody 67905-1-IgDownload protocol
IHC protocol for METTL7A antibody 67905-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols