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验证数据展示

  • WB analysis using 84257-1-RR (same clone as 84257-1-PBS)

    WB analysis using 84257-1-RR (same clone as 84257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84257-1-RR (same clone as 84257-1-PBS)

    IF Staining of MCF-7 using 84257-1-RR (same clone as 84257-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01163-3

    Cytometric bead array standard curve of MP01163-3

    Cytometric bead array standard curve of MP01163-3, METTL2B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84257-1-PBS. Detection antibody: 84257-3-PBS. Standard: Ag10410. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84257-1-RR

    Affinity and Kinetic Characterization of 84257-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

METTL2 Recombinant antibody, PBS Only (Capture)

METTL2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84257-1-PBS

产品说明书

CloneNo. 241490A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84257-1-PBS targets METTL2 as part of a matched antibody pair:

MP01163-3: 84257-1-PBS capture and 84257-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 METTL2 fusion protein Ag10410 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 2B
别名 METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B
计算分子量 313 aa, 36 kDa
观测分子量36~43 kDa
GenBank蛋白编号BC107586
基因名称 METTL2B
Gene ID (NCBI) 55798
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6P1Q9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.