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验证数据展示

  • WB analysis using 84257-1-RR

    WB analysis using 84257-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84257-1-RR

    IF Staining of MCF-7 using 84257-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84257-1-RR

    Affinity and Kinetic Characterization of 84257-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

METTL2 Recombinant antibody

METTL2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84257-1-RR

产品说明书

CloneNo. 241490A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, PC-3 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84257-1-RR targets METTL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 METTL2 fusion protein Ag10410 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 2B
别名 METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B
计算分子量 313 aa, 36 kDa
观测分子量36~43 kDa
GenBank蛋白编号BC107586
基因名称 METTL2B
Gene ID (NCBI) 55798
RRIDAB_3671804
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6P1Q9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.

实验方案

Product Specific Protocols
WB protocol for METTL2 antibody 84257-1-RRDownload protocol
IF protocol for METTL2 antibody 84257-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols