验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, PC-3 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84257-1-RR targets METTL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10410 Product name: Recombinant human METTL2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC107586 Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 2B |
| 别名 | METTL2B, 241490A3, EC:2.1.1.-, methyltransferase like 2B, Methyltransferase-like protein 2B |
| 计算分子量 | 313 aa, 36 kDa |
| 观测分子量 | 36~43 kDa |
| GenBank蛋白编号 | BC107586 |
| 基因名称 | METTL2B |
| Gene ID (NCBI) | 55798 |
| RRID | AB_3671804 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6P1Q9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL2 antibody 84257-1-RR | Download protocol |
| WB protocol for METTL2 antibody 84257-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
