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验证数据展示

  • WB analysis using 68473-1-Ig

    WB analysis using 68473-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68473-1-Ig (MEN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68473-1-Ig

    IHC staining of mouse brain using 68473-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68473-1-Ig

    IHC staining of mouse brain using 68473-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MEN1 Monoclonal antibody

MEN1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68473-1-Ig

产品说明书

CloneNo. 3A6A11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

3A6A11, MEAI, Menin, Menin 1, SCG2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, U2OS cells, LNCaP cells, K-562 cells, Jurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68473-1-Ig targets MEN1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MEN1 fusion protein Ag7566 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 multiple endocrine neoplasia I
别名 3A6A11, MEAI, Menin, Menin 1, SCG2
计算分子量 68 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC002544
基因名称 MEN1
Gene ID (NCBI) 4221
RRIDAB_3085184
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00255
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. It is located in nucleus and ubiquitous expression in spleen and lymph node. The calculated molecular weight of MEN1 is 68 kDa. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice.

实验方案

Product Specific Protocols
WB protocol for MEN1 antibody 68473-1-IgDownload protocol
IHC protocol for MEN1 antibody 68473-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols