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CoraLite® Plus 647-conjugated MEK6 Polyclonal antibody

MEK6 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-12745

Print datasheet

Synonyms

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3

验证数据展示

  • FC experiment of HeLa using CL647-12745

    FC experiment of HeLa using CL647-12745

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MEK6 (CL647-12745) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12745-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12745 targets MEK6 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen MEK6 fusion protein Ag3706 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 6
Synonyms MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3
Calculated Molecular Weight 334 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012009
Gene Symbol MAP2K6
Gene ID (NCBI) 5608
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52564
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MEK6 antibody CL647-12745Download protocol
Standard Protocols
Click here to view our Standard Protocols