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CoraLite® Plus 488-conjugated MEK6 Polyclonal antibody

MEK6 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-12745

Print datasheet

Synonyms

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3

验证数据展示

  • IF Staining of HepG2 using CL488-12745

    IF Staining of HepG2 using CL488-12745

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MEK6 antibody (CL488-12745) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-12745

    FC experiment of HepG2 using CL488-12745

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MEK6 (CL488-12745) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12745-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-12745 targets MEK6 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MEK6 fusion protein Ag3706 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 6
Synonyms MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3
Calculated Molecular Weight 334 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012009
Gene Symbol MAP2K6
Gene ID (NCBI) 5608
RRIDAB_2919059
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52564
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MEK6 antibody CL488-12745Download protocol
FC protocol for CL Plus 488 MEK6 antibody CL488-12745Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Adv Biol (Weinh)

Single-Cell RNA Transcriptome of the Human Endometrium Reveals Epithelial Characterizations Associated with Recurrent Implantation Failure

Authors - Hong Zhang
View Article