CoraLite® Plus 488-conjugated MEK1 Monoclonal antibody
MEK1 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3F6D7
验证数据展示
经过测试的应用
Positive IF detected in | A431 cells |
Positive FC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67872-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67872 targets MEK1 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | Human, rat, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase kinase 1 |
Synonyms | ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, PRKMK1 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 40-50 kDa |
GenBank Accession Number | BC139729 |
Gene Symbol | MAP2K1 |
Gene ID (NCBI) | 5604 |
ENSEMBL Gene ID | ENSG00000169032 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q02750 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 MEK1 antibody CL488-67872 | Download protocol |
FC protocol for CL Plus 488 MEK1 antibody CL488-67872 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |