验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, Calyculin A treated HeLa cells, NIH/3T3 cells, HSC-T6 cells, Calyculin A treated PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67872-1-Ig targets MEK1 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, rat, mouse |
| 文献引用反应性 | rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 1 |
| 别名 | ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC139729 |
| 基因名称 | MEK1 |
| Gene ID (NCBI) | 5604 |
| ENSEMBL Gene ID | ENSG00000169032 |
| RRID | AB_2918630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q02750 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEK1 antibody 67872-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
