- Featured Product
- KD/KO Validated
MEK1 Monoclonal antibody
MEK1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3F6D7
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, Calyculin A treated HeLa cells, NIH/3T3 cells, HSC-T6 cells, Calyculin A treated PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67872-1-Ig targets MEK1 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase 1 |
| Synonyms | ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC139729 |
| Gene Symbol | MAP2K1 |
| Gene ID (NCBI) | 5604 |
| ENSEMBL Gene ID | ENSG00000169032 |
| RRID | AB_2918630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q02750 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEK1 antibody 67872-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Jia-Wei-Si-Miao-Yong-An Fang stimulates the healing of acute radiation-induced cutaneous wounds through MAPK/ERK pathway |
