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验证数据展示

  • WB analysis using 85801-5-RR

    WB analysis using 85801-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85801-5-RR

    IF Staining of HeLa using 85801-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (85801-5-RR, Clone: 250068B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 85801-5-RR

    IHC staining of mouse brain using 85801-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEF2D antibody included
  • Affinity and Kinetic Characterization of 85801-5-RR

    Affinity and Kinetic Characterization of 85801-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85801-5-RR against Human MEF2D were performed. The affinity constant is 1.99 nM.

MEF2D Recombinant antibody

MEF2D Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85801-5-RR

产品说明书

CloneNo. 250068B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Myocyte-specific enhancer factor 2D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, THP-1 cells, K-562 cells, U2OS cells, C2C12 cells, C6 cells, U266 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MEF2D Ready-To-Use IHC Kit. MEF2D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85801-5-RR targets MEF2D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MEF2D fusion protein Ag5700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myocyte enhancer factor 2D
别名 Myocyte-specific enhancer factor 2D
计算分子量 56 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC040949
基因名称 MEF2D
Gene ID (NCBI) 4209
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.

实验方案

Product Specific Protocols
WB protocol for MEF2D antibody 85801-5-RRDownload protocol
IHC protocol for MEF2D antibody 85801-5-RRDownload protocol
IF protocol for MEF2D antibody 85801-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols