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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

    WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85801-5-RR (same clone as 85801-5-PBS)

    IF Staining of HeLa using 85801-5-RR (same clone as 85801-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (85801-5-RR, Clone: 250068B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

    IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MEF2D antibody included
  • Affinity and Kinetic Characterization of 85801-5-RR

    Affinity and Kinetic Characterization of 85801-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85801-5-RR against Human MEF2D were performed. The affinity constant is 1.99 nM.

MEF2D Recombinant antibody, PBS Only

MEF2D Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85801-5-PBS

产品说明书

CloneNo. 250068B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Myocyte-specific enhancer factor 2D

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MEF2D Ready-To-Use IHC Kit. MEF2D primary antibody included.

产品信息

85801-5-PBS targets MEF2D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MEF2D fusion protein Ag5700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myocyte enhancer factor 2D
别名 Myocyte-specific enhancer factor 2D
计算分子量 56 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC040949
基因名称 MEF2D
Gene ID (NCBI) 4209
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.