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验证数据展示

  • FC experiment of HeLa using CL488-67038

    FC experiment of HeLa using CL488-67038

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED30 (CL488-67038, Clone:1D7C4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MED30 Monoclonal antibody

MED30 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67038

产品说明书

CloneNo. 1D7C4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, pig

应用

FC (Intra)

别名

Thyroid hormone receptor-associated protein complex 25 kDa component, Thyroid hormone receptor-associated protein 6, THRAP6, Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67038-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67038 targets MED30 in FC (Intra) applications and shows reactivity with Human, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat, pig
免疫原

CatNo: Ag10235

Product name: Recombinant human MED30 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-178 aa of BC008226

Sequence: MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGTYQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mediator complex subunit 30
别名 Thyroid hormone receptor-associated protein complex 25 kDa component, Thyroid hormone receptor-associated protein 6, THRAP6, Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30
计算分子量 178 aa, 20 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC008226
基因名称 MED30
Gene ID (NCBI) 90390
RRIDAB_2934515
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96HR3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MED30 antibody CL488-67038Download protocol
Standard Protocols
Click here to view our Standard Protocols