验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, pig brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67038-1-Ig targets MED30 in WB, ELISA applications and shows reactivity with Human, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rat, pig |
| 免疫原 |
CatNo: Ag10235 Product name: Recombinant human MED30 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-178 aa of BC008226 Sequence: MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGTYQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 30 |
| 别名 | MED30, mediator complex subunit 30, THRAP6, TRAP25 |
| 计算分子量 | 178 aa, 20 kDa |
| 观测分子量 | 20-25 kDa |
| GenBank蛋白编号 | BC008226 |
| 基因名称 | MED30 |
| Gene ID (NCBI) | 90390 |
| RRID | AB_2882352 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96HR3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED30 antibody 67038-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
