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ME2 Polyclonal antibody

ME2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24944-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

Malic enzyme 2, ME2, NAD ME

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24944-1-AP targets ME2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human
文献引用反应性human
免疫原 ME2 fusion protein Ag20741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 malic enzyme 2, NAD(+)-dependent, mitochondrial
别名 Malic enzyme 2, ME2, NAD ME
计算分子量 65 kDa
观测分子量 54 kDa, 65 kDa
GenBank蛋白编号BC000147
基因名称 ME2
Gene ID (NCBI) 4200
RRIDAB_2879813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23368
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

实验方案

Product Specific Protocols
WB protocol for ME2 antibody 24944-1-APDownload protocol
IHC protocol for ME2 antibody 24944-1-APDownload protocol
IP protocol for ME2 antibody 24944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Authors - Qifan Hu
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
humanWB

Cell Death Differ

SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration

Authors - Peng Teng
  • KD Validated
humanWB,IP

Nat Commun

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis

Authors - Taiqi Chen
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