验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells, K-562 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human pancreas cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24944-1-AP targets ME2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IP |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | ME2 fusion protein Ag20741 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | malic enzyme 2, NAD(+)-dependent, mitochondrial |
别名 | Malic enzyme 2, ME2, NAD ME |
计算分子量 | 65 kDa |
观测分子量 | 54 kDa, 65 kDa |
GenBank蛋白编号 | BC000147 |
基因名称 | ME2 |
Gene ID (NCBI) | 4200 |
RRID | AB_2879813 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P23368 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ME2 antibody 24944-1-AP | Download protocol |
IHC protocol for ME2 antibody 24944-1-AP | Download protocol |
IP protocol for ME2 antibody 24944-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Cell Death Differ SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration
| ||
Nat Commun AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis |