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- KD/KO Validated
ME2 Polyclonal antibody
ME2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24944-1-AP targets ME2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ME2 fusion protein Ag20741 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | malic enzyme 2, NAD(+)-dependent, mitochondrial |
| Synonyms | Malic enzyme 2, ME2, NAD ME |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 54 kDa, 65 kDa |
| GenBank Accession Number | BC000147 |
| Gene Symbol | ME2 |
| Gene ID (NCBI) | 4200 |
| RRID | AB_2879813 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23368 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ME2 antibody 24944-1-AP | Download protocol |
| IHC protocol for ME2 antibody 24944-1-AP | Download protocol |
| IP protocol for ME2 antibody 24944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma | ||
Cell Death Differ SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration
| ||
Nat Commun AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis |
