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验证数据展示

  • IF Staining of HeLa using CL488-83496

    IF Staining of HeLa using CL488-83496

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MDC1 antibody (CL488-83496, Clone: 240469D5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83496

    FC experiment of HeLa using CL488-83496

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MDC1 Recombinant antibody (CL488-83496, Clone:240469D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated MDC1 Recombinant antibody

MDC1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83496

产品说明书

CloneNo. 240469D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

NFBD1, NFBD 1, MDC 1, 240469D5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MDC1 Ready-To-Use IHC Kit. MDC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83496-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83496 targets MDC1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 MDC1 fusion protein Ag20135 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator of DNA damage checkpoint 1
别名 NFBD1, NFBD 1, MDC 1, 240469D5
计算分子量 2089 aa, 227 kDa
观测分子量260-320 kDa
GenBank蛋白编号BC152556
基因名称 MDC1
Gene ID (NCBI) 9656
RRIDAB_3673259
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14676
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MDC1 antibody CL488-83496Download protocol
FC protocol for CL Plus 488 MDC1 antibody CL488-83496Download protocol
Standard Protocols
Click here to view our Standard Protocols