验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, NIH/3T3 cells, SW 480 cells, U2OS cells, HEK-293T cells, RAW 264.7 cells, HSC-T6 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87212-1-RR targets MCM8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8430 Product name: Recombinant human MCM8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 380-735 aa of BC008830 Sequence: SGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | minichromosome maintenance complex component 8 |
| 别名 | C20orf154, DNA helicase MCM8, EC:3.6.4.12, MCM 8, REC |
| 计算分子量 | 880 aa, 98 kDa |
| 观测分子量 | 90-95 kDa |
| GenBank蛋白编号 | BC008830 |
| 基因名称 | MCM8 |
| Gene ID (NCBI) | 84515 |
| RRID | AB_3745410 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UJA3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MCM8 antibody 87212-1-RR | Download protocol |
| WB protocol for MCM8 antibody 87212-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
