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验证数据展示

  • WB analysis using 87212-1-RR (same clone as 87212-1-PBS)

    WB analysis using 87212-1-RR (same clone as 87212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87212-1-RR (MCM8 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87212-1-PBS in a different storage buffer formulation.

  • WB analysis using 87212-1-RR (same clone as 87212-1-PBS)

    WB analysis using 87212-1-RR (same clone as 87212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87212-1-RR (MCM8 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87212-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 87212-1-RR (same clone as 87212-1-PBS)

    IF Staining of NIH/3T3 using 87212-1-RR (same clone as 87212-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MCM8 antibody (87212-1-RR, Clone: 252460F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87212-1-PBS in a different storage buffer formulation.

MCM8 Recombinant monoclonal antibody, PBS Only

MCM8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87212-1-PBS

产品说明书

CloneNo. 252460F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

C20orf154, DNA helicase MCM8, EC:3.6.4.12, MCM 8, REC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87212-1-PBS targets MCM8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8430

Product name: Recombinant human MCM8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 380-735 aa of BC008830

Sequence: SGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 minichromosome maintenance complex component 8
别名 C20orf154, DNA helicase MCM8, EC:3.6.4.12, MCM 8, REC
计算分子量 880 aa, 98 kDa
观测分子量 90-95 kDa
GenBank蛋白编号BC008830
基因名称 MCM8
Gene ID (NCBI) 84515
RRIDAB_3745410
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UJA3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis.

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