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验证数据展示

  • IF Staining of HepG2 using CL594-67989

    IF Staining of HepG2 using CL594-67989

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 MCM6 antibody (CL594-67989, Clone: 2E12H1 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-67989

    FC experiment of HepG2 using CL594-67989

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MCM6 (CL594-67989, Clone:2E12H1) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated MCM6 Monoclonal antibody

MCM6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67989

产品说明书

CloneNo. 2E12H1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

P105MCM, Mis5, MCG40308, EC:3.6.4.12, 2E12H1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCM6 Ready-To-Use IHC Kit. MCM6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67989-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67989 targets MCM6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MCM6 fusion protein Ag4354 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 6
别名 P105MCM, Mis5, MCG40308, EC:3.6.4.12, 2E12H1
计算分子量 821 aa, 93 kDa
观测分子量105 kDa
GenBank蛋白编号BC032374
基因名称 MCM6
Gene ID (NCBI) 4175
RRIDAB_2934789
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14566
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 MCM6 antibody CL594-67989Download protocol
FC protocol for CL594 MCM6 antibody CL594-67989Download protocol
Standard Protocols
Click here to view our Standard Protocols