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CoraLite®555-conjugated MARCKS Polyclonal antibody

MARCKS Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite®555 Fluorescent Dye

Cat No : CL555-20661

Print datasheet

Synonyms

80K L, 80K L protein, FLJ14368, FLJ90045, MACS, MARCKS, PKCSL, PRKCSL

验证数据展示

  • IF Staining of mouse brain using CL555-20661

    IF Staining of mouse brain using CL555-20661

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®555 MARCKS antibody (CL555-20661) at dilution of 1:200.

  • FC experiment of HeLa using CL555-20661

    FC experiment of HeLa using CL555-20661

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human MARCKS (CL555-20661) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse brain tissue
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20661-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-20661 targets MARCKS in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myristoylated alanine-rich protein kinase C substrate
Synonyms 80K L, 80K L protein, FLJ14368, FLJ90045, MACS, MARCKS, PKCSL, PRKCSL
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberNM_002356
Gene Symbol MARCKS
Gene ID (NCBI) 4082
RRIDAB_2919638
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29966
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

实验方案

Product Specific Protocols
IF protocol for CL555 MARCKS antibody CL555-20661Download protocol
Standard Protocols
Click here to view our Standard Protocols