验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, IMR-32 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells, SK-N-SH cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20661-1-AP targets MARCKS in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myristoylated alanine-rich protein kinase C substrate |
| 别名 | 80K L, 80K L protein, 80KL, 80K-L protein, MACS |
| 计算分子量 | 32 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | NM_002356 |
| 基因名称 | MARCKS |
| Gene ID (NCBI) | 4082 |
| RRID | AB_2878719 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29966 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MARCKS antibody 20661-1-AP | Download protocol |
| IHC protocol for MARCKS antibody 20661-1-AP | Download protocol |
| IP protocol for MARCKS antibody 20661-1-AP | Download protocol |
| WB protocol for MARCKS antibody 20661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Oncoprotein DJ-1 interacts with mTOR complexes to effect transcription factor Hif1α-dependent expression of collagen I (α2) during renal fibrosis. | ||
J Proteomics Proteomic profile of human dental follicle stem cells and apical papilla stem cells. | ||
Int J Oncol Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma. | ||
Front Med (Lausanne) Enhanced understanding of cinnamaldehyde's therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect | ||
J Vet Med Sci A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells | ||
bioRxiv Sex specific disruptions in Protein Kinase Cγ signaling in a mouse model of Spinocerebellar Ataxia Type 14 |
