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验证数据展示

  • IHC staining of human ovary tumor using Biotin-23382

    IHC staining of human ovary tumor using Biotin-23382

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-23382 (MAN1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using Biotin-23382

    IHC staining of human ovary tumor using Biotin-23382

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-23382 (MAN1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Biotin-conjugated MAN1A1 Polyclonal antibody

MAN1A1 Polyclonal Antibody for IHC, ELISA
Cat No. Biotin-23382

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-23382 targets MAN1A1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAN1A1 fusion protein Ag20060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosidase, alpha, class 1A, member 1
别名 Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
计算分子量 653 aa, 73 kDa
观测分子量 68 kDa
GenBank蛋白编号BC065827
基因名称 MAN1A1
Gene ID (NCBI) 4121
偶联类型 Biotin
最大激发/发射波长-
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33908
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

实验方案

Product Specific Protocols
IHC protocol for Biotin MAN1A1 antibody Biotin-23382Download protocol
Standard Protocols
Click here to view our Standard Protocols