Biotin-conjugated MAN1A1 Polyclonal antibody

MAN1A1 Polyclonal Antibody for IHC, ELISA
Cat No. Biotin-23382

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格价格库存


经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-23382 targets MAN1A1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20060

Product name: Recombinant human MAN1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-367 aa of BC065827

Sequence: VLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEEIEARKGQANCPGSSSSCEVEIQMMKHAWNNYKGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKRWMCNIPRAMEATI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosidase, alpha, class 1A, member 1
别名 Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
计算分子量 653 aa, 73 kDa
观测分子量 68 kDa
GenBank蛋白编号BC065827
基因名称 MAN1A1
Gene ID (NCBI) 4121
偶联类型 Biotin
最大激发/发射波长-
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33908
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

实验方案

Product Specific Protocols
IHC protocol for Biotin MAN1A1 antibody Biotin-23382Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...