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验证数据展示

  • WB analysis using 23382-1-AP

    WB analysis using 23382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23382-1-AP (MAN1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 23382-1-AP

    IF Staining of HepG2 using 23382-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (23382-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 23382-1-AP

    FC experiment of HepG2 using 23382-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAN1A1 Polyclonal antibody (23382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of HepG2 using 23382-1-AP

    IP experiment of HepG2 using 23382-1-AP

    IP result of anti-MAN1A1 (IP:23382-1-AP, 4ug; Detection:23382-1-AP 1:10000) with HepG2 cells lysate 2000 ug.

  • IHC staining of human ovary cancer using 23382-1-AP

    IHC staining of human ovary cancer using 23382-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23382-1-AP (MAN1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAN1A1 Polyclonal antibody

MAN1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 23382-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
Biotin
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23382-1-AP targets MAN1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20060

Product name: Recombinant human MAN1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-367 aa of BC065827

Sequence: VLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEEIEARKGQANCPGSSSSCEVEIQMMKHAWNNYKGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKRWMCNIPRAMEATI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosidase, alpha, class 1A, member 1
别名 Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
计算分子量 653 aa, 73 kDa
观测分子量 68 kDa
GenBank蛋白编号BC065827
基因名称 MAN1A1
Gene ID (NCBI) 4121
RRIDAB_2935458
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33908
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

实验方案

Product Specific Protocols
FC protocol for MAN1A1 antibody 23382-1-APDownload protocol
IF protocol for MAN1A1 antibody 23382-1-APDownload protocol
IHC protocol for MAN1A1 antibody 23382-1-APDownload protocol
IP protocol for MAN1A1 antibody 23382-1-APDownload protocol
WB protocol for MAN1A1 antibody 23382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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