MAN1A1 Polyclonal antibody
MAN1A1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | MCF-7 cells |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23382-1-AP targets MAN1A1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Tested Reactivity | human, mouse, rat |
Immunogen | MAN1A1 fusion protein Ag20060 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mannosidase, alpha, class 1A, member 1 |
Synonyms | Alpha 1,2 mannosidase IA, HUMM3, HUMM9, Man(9) alpha mannosidase, MAN1A1, MAN9, Man9 mannosidase |
Calculated Molecular Weight | 653 aa, 73 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC065827 |
Gene Symbol | MAN1A1 |
Gene ID (NCBI) | 4121 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P33908 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).
实验方案
Product Specific Protocols | |
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WB protocol for MAN1A1 antibody 23382-1-AP | Download protocol |
IF protocol for MAN1A1 antibody 23382-1-AP | Download protocol |
IP protocol for MAN1A1 antibody 23382-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |