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验证数据展示

  • IF Staining of H9C2 using CL488-84440-5

    IF Staining of H9C2 using CL488-84440-5

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CoraLite® Plus 488 M-cadherin antibody (CL488-84440-5, Clone: 241837C10 ) at dilution of 1:200.

  • IF Staining of C2C12 using CL488-84440-5

    IF Staining of C2C12 using CL488-84440-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CoraLite® Plus 488 M-cadherin antibody (CL488-84440-5, Clone: 241837C10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated M-cadherin Recombinant antibody

M-cadherin Recombinant Antibody for IF/ICC
Cat No. CL488-84440-5

产品说明书

CloneNo. 241837C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inH9C2 cells, C2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 84440-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84440-5 targets M-cadherin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 M-cadherin fusion protein Ag25763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 15, type 1, M-cadherin (myotubule)
别名 CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14
计算分子量 89 kDa
观测分子量90-130 kDa
GenBank蛋白编号BC008951
基因名称 M-cadherin
Gene ID (NCBI) 1013
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55291
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 M-cadherin antibody CL488-84440-5Download protocol
Standard Protocols
Click here to view our Standard Protocols