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验证数据展示

  • WB analysis of mouse cerebellum using 84440-5-RR (same clone as 84440-5-PBS)

    WB analysis of mouse cerebellum using 84440-5-RR (same clone as 84440-5-PBS)

    mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 84440-5-RR (CDH15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 84440-5-RR (same clone as 84440-5-PBS)

    IHC staining of mouse skeletal muscle using 84440-5-RR (same clone as 84440-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84440-5-RR (M-cadherin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 84440-5-RR (same clone as 84440-5-PBS)

    IF Staining of C2C12 using 84440-5-RR (same clone as 84440-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CDH15 antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 84440-5-RR (same clone as 84440-5-PBS)

    IF Staining of mouse skeletal muscle using 84440-5-RR (same clone as 84440-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using M-cadherin antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 84440-5-RR (same clone as 84440-5-PBS)

    IF Staining of H9C2 using 84440-5-RR (same clone as 84440-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using M-cadherin antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84440-5-RR

    Affinity and Kinetic Characterization of 84440-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84440-5-RR against Human M-cadherin were performed. The affinity constant is below 1 pM.

M-cadherin Recombinant monoclonal antibody, PBS Only

M-cadherin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 84440-5-PBS

产品说明书

CloneNo. 241837C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84440-5-PBS targets M-cadherin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25763

Product name: Recombinant human CDH15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-450 aa of BC008951

Sequence: AFLQEAFTGRVLEGAVPGTYVTRAEATDADDPETDNAALRFSILQQGSPELFSIDELTGEIRTVQVGLDREVVAVYNLTLQVADMSGDGLTATASAIITLDDINDNAPEFTRDEFFMEAIEAVSGVDVGRLEVEDRDLPGSPNWVARFTILEGDPDGQFTIRTDPKTNEGVLSIVKALDYESCEHYELKVSVQNEAPLQAAALRAERGQAKVRVHVQDTNEPPVFQENPLRTSLAEGAPPGTLVATFSARDPDTEQLQRLSYSKDYDPEDWLQVDAATGRIQTQHVLSPASPFLKGGWYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 15, type 1, M-cadherin (myotubule)
别名 CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14
计算分子量 89 kDa
观测分子量90-130 kDa
GenBank蛋白编号BC008951
基因名称 M-cadherin
Gene ID (NCBI) 1013
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP55291
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).

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