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验证数据展示

  • FC experiment of HeLa using CL647-14175

    FC experiment of HeLa using CL647-14175

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Liprin Alpha 1 (CL647-14175) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated Liprin Alpha 1 Polyclonal antibody

Liprin Alpha 1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-14175

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

FLJ41337, FLJ42630, FLJ43474, LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPFIA1 Ready-To-Use IHC Kit. PPFIA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14175-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14175 targets Liprin Alpha 1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Liprin Alpha 1 fusion protein Ag5381 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
别名 FLJ41337, FLJ42630, FLJ43474, LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1
计算分子量 1202 aa, 136 kDa
观测分子量 160 kDa
GenBank蛋白编号BC034046
基因名称 Liprin Alpha 1
Gene ID (NCBI) 8500
RRIDAB_2934890
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13136
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Liprin Alpha 1 antibody CL647-14175Download protocol
Standard Protocols
Click here to view our Standard Protocols