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- KD/KO Validated
Liprin Alpha 1 Polyclonal antibody
Liprin Alpha 1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
FC, IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PPFIA1 Ready-To-Use IHC Kit. PPFIA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14175-1-AP targets Liprin Alpha 1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | Liprin Alpha 1 fusion protein Ag5381 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 |
| Synonyms | LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1 |
| Calculated Molecular Weight | 1202 aa, 136 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC034046 |
| Gene Symbol | PPFIA1 |
| Gene ID (NCBI) | 8500 |
| RRID | AB_2171592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13136 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IHC protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IF protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IP protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| FC protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity. | ||
Nat Commun PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
| ||
Elife Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion. | ||
Mol Oncol Liprin-α1 contributes to oncogenic MAPK signaling by counteracting ERK activity
| ||
Stem Cells Transl Med Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix. | ||
J Cell Sci Liprin-α1, ERC1 and LL5 identify a polarized, dynamic compartment implicated in cell migration.
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