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验证数据展示

  • WB analysis using 14175-1-AP

    WB analysis using 14175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin Alpha 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14175-1-AP

    IHC staining of human prostate cancer using 14175-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPFIA1 antibody included
  • IHC staining of human prostate cancer using 14175-1-AP

    IHC staining of human prostate cancer using 14175-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPFIA1 antibody included
  • IP experiment of MCF-7 using 14175-1-AP

    IP experiment of MCF-7 using 14175-1-AP

    IP result of anti-Liprin Alpha 1 (IP:14175-1-AP, 4ug; Detection:14175-1-AP 1:500) with MCF-7 cells lysate 3200ug.

  • WB analysis of MCF-7 using 14175-1-AP

    WB analysis of MCF-7 using 14175-1-AP

    WB result of Liprin Alpha 1 antibody (14175-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Liprin Alpha 1 transfected MCF-7 cells.

  • IF Staining of HepG2 using 14175-1-AP

    IF Staining of HepG2 using 14175-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Liprin Alpha 1 antibody (14175-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of A549 using 14175-1-AP

    IF Staining of A549 using 14175-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Liprin Alpha 1 antibody (14175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

Liprin Alpha 1 Polyclonal antibody

Liprin Alpha 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14175-1-AP

产品说明书

引用文献(38)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

PPFIA1, LAR interacting protein 1, LAR-interacting protein 1, LIP 1, LIP.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy PPFIA1 Ready-To-Use IHC Kit. PPFIA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14175-1-AP targets Liprin Alpha 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, monkey
免疫原

CatNo: Ag5381

Product name: Recombinant human PPFIA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-354 aa of BC034046

Sequence: MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
别名 PPFIA1, LAR interacting protein 1, LAR-interacting protein 1, LIP 1, LIP.1
计算分子量 1202 aa, 136 kDa
观测分子量 140-160 kDa
GenBank蛋白编号BC034046
基因名称 Liprin Alpha 1
Gene ID (NCBI) 8500
RRIDAB_2171592
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13136
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

实验方案

Product Specific Protocols
IF protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
IHC protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
IP protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
WB protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.

Authors - Giulia Mana
  • KD Validated
View Article
humanIF

Nat Commun

Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.

Authors - Xingqiao Xie
View Article

Neuron

Neurexin-1γ drives synaptic transmission through structural integration of neurotransmitter release machinery and postsynaptic receptor nanodomains.

Authors - Nirmala Padmanabhan
View Article
mouseWB

Nat Chem Biol

Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.

Authors - Matthew E Griffin
View Article
humanWB,IF

Cell Commun Signal

Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.

Authors - Henna Pehkonen
  • KD Validated
View Article
mouse,humanIF

Diabetologia

Capillary contact points determine beta cell polarity, control secretion and are disrupted in the db/db mouse model of diabetes.

Authors - Dillon Jevon
View Article
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