验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy LYN Ready-To-Use IHC Kit. LYN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60211-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60211 targets LYN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | LYN fusion protein Ag12723 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-yes-1 Yamaguchi sarcoma viral related oncogene homolog |
| 别名 | Tyrosine-protein kinase Lyn, Tyrosine protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, JTK8, EC:2.7.10.2 |
| 计算分子量 | 512 aa, 59 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC075002 |
| 基因名称 | LYN |
| Gene ID (NCBI) | 4067 |
| RRID | AB_2919237 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07948 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LYN antibody CL488-60211 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |