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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85628-1-RR (same clone as 85628-1-PBS)

    WB analysis using 85628-1-RR (same clone as 85628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85628-1-RR (LRAT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85628-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85628-1-RR

    Affinity and Kinetic Characterization of 85628-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85628-1-RR against Human LRAT were performed. The affinity constant is below 1 pM.

LRAT Recombinant antibody, PBS Only

LRAT Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85628-1-PBS

产品说明书

CloneNo. 243058B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LRAT Ready-To-Use IHC Kit. LRAT primary antibody included.

产品信息

85628-1-PBS targets LRAT in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LRAT fusion protein Ag3556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
别名 EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase
计算分子量 230 aa, 26 kDa
观测分子量 26 kDa, 50-54 kDa
GenBank蛋白编号BC031053
基因名称 LRAT
Gene ID (NCBI) 9227
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95237
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.