验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85628-1-RR targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3556 Product name: Recombinant human LRAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-201 aa of BC031053 Sequence: EDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) |
| 别名 | EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase |
| 计算分子量 | 230 aa, 26 kDa |
| 观测分子量 | 26 kDa, 50-54 kDa |
| GenBank蛋白编号 | BC031053 |
| 基因名称 | LRAT |
| Gene ID (NCBI) | 9227 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95237 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRAT antibody 85628-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
