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验证数据展示

  • WB analysis using 86117-3-RR

    WB analysis using 86117-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86117-3-RR

    WB analysis using 86117-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86117-3-RR

    IF Staining of HeLa using 86117-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPP antibody (86117-3-RR, Clone: 250773C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

LPP Recombinant antibody

LPP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86117-3-RR

产品说明书

CloneNo. 250773C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse colon tissue, HEK-293 cells, MCF-7 cells, rat colon tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LPP Ready-To-Use IHC Kit. LPP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86117-3-RR targets LPP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LPP fusion protein Ag19205 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 LIM domain containing preferred translocation partner in lipoma
别名 LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner
计算分子量 612 aa, 66 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC130584
基因名称 LPP
Gene ID (NCBI) 4026
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93052
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).

实验方案

Product Specific Protocols
WB protocol for LPP antibody 86117-3-RRDownload protocol
IF protocol for LPP antibody 86117-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols