验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse colon tissue, HEK-293 cells, MCF-7 cells, rat colon tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86117-3-RR targets LPP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | LPP fusion protein Ag19205 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | LIM domain containing preferred translocation partner in lipoma |
别名 | LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner |
计算分子量 | 612 aa, 66 kDa |
观测分子量 | 70-75 kDa |
GenBank蛋白编号 | BC130584 |
基因名称 | LPP |
Gene ID (NCBI) | 4026 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q93052 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).
实验方案
Product Specific Protocols | |
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WB protocol for LPP antibody 86117-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |