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验证数据展示

  • WB analysis using 86117-3-RR (same clone as 86117-3-PBS)

    WB analysis using 86117-3-RR (same clone as 86117-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86117-3-PBS in a different storage buffer formulation.

  • WB analysis using 86117-3-RR (same clone as 86117-3-PBS)

    WB analysis using 86117-3-RR (same clone as 86117-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86117-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86117-3-RR (same clone as 86117-3-PBS)

    IF Staining of HeLa using 86117-3-RR (same clone as 86117-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPP antibody (86117-3-RR, Clone: 250773C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86117-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86117-3-RR

    Affinity and Kinetic Characterization of 86117-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86117-3-RR against Human LPP were performed. The affinity constant is 1.18 nM.

LPP Recombinant monoclonal antibody, PBS Only

LPP Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86117-3-PBS

产品说明书

CloneNo. 250773C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LPP Ready-To-Use IHC Kit. LPP primary antibody included.

产品信息

86117-3-PBS targets LPP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19205

Product name: Recombinant human LPP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC130584

Sequence: MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPPPPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPRPPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASYTTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPGLQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 LIM domain containing preferred translocation partner in lipoma
别名 LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner
计算分子量 612 aa, 66 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC130584
基因名称 LPP
Gene ID (NCBI) 4026
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ93052
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).

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