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LPIN1 Monoclonal antibody

LPIN1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G11B10

Cat No : 68124-1-Ig

Print datasheet

Synonyms

DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1

验证数据展示

  • WB analysis using 68124-1-Ig

    WB analysis using 68124-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68124-1-Ig (LPIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IHC staining of human liver cancer using 68124-1-Ig

    IHC staining of human liver cancer using 68124-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPIN1 antibody included
  • IF Staining of NIH/3T3 using 68124-1-Ig

    IF Staining of NIH/3T3 using 68124-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using LPIN1 antibody (68124-1-Ig, Clone: 3G11B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy LPIN1 Ready-To-Use IHC Kit. LPIN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68124-1-Ig targets LPIN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Rat, Mouse
Cited Reactivityrat
Immunogen LPIN1 fusion protein Ag25813 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name lipin 1
Synonyms DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1
Calculated Molecular Weight 890 aa, 99 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC030537
Gene Symbol LPIN1
Gene ID (NCBI) 23175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).

实验方案

Product Specific Protocols
WB protocol for LPIN1 antibody 68124-1-IgDownload protocol
IHC protocol for LPIN1 antibody 68124-1-IgDownload protocol
IF protocol for LPIN1 antibody 68124-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Eur J Pharmacol

Exploring the effects of epigallocatechin gallate on lipid metabolism in the rat steatotic liver during normothermic machine perfusion: Insights from lipidomics and RNA sequencing

Authors - Shuxuan Li
View Article