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EDG2 Polyclonal antibody

EDG2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20442-1-AP

Print datasheet

Synonyms

edg 2, EDG2, Gpcr26, GPR26, LPA 1, LPA receptor 1, LPA1, LPAR1, Mrec1.3, rec.1.3, vzg 1, VZG1

验证数据展示

  • WB analysis of Jurkat using 20442-1-AP

    WB analysis of Jurkat using 20442-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of multi-cells/tissue using 20442-1-AP

    WB analysis of multi-cells/tissue using 20442-1-AP

    Multi-cells/tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20442-1-AP

    IHC staining of human brain using 20442-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20442-1-AP

    IHC staining of human brain using 20442-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, A375 cells, HeLa cells, mouse brain tissue, multi-cells/tissue, A549 cells, A2780 cells, NIH3T3 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20442-1-AP targets EDG2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen EDG2 fusion protein Ag14253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysophosphatidic acid receptor 1
Synonyms edg 2, EDG2, Gpcr26, GPR26, LPA 1, LPA receptor 1, LPA1, LPAR1, Mrec1.3, rec.1.3, vzg 1, VZG1
Calculated Molecular Weight 364 aa, 41 kDa
Observed Molecular Weight 41-50 kDa
GenBank Accession NumberBC030615
Gene Symbol LPAR1
Gene ID (NCBI) 1902
RRIDAB_11183048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EDG2 (also known as LPA1) is a G-protein coupled receptor for lysophosphatidic acid (LPA), a potent motility inducing factor, which is a major component of serum (PMID: 19415462). EDG2 is widely expressed in normal tissue during growth and development. In the context of cancer, several studies have suggested that EDG2 expression in tumors is often similar to that shown in normal tissue (PMID: 17496233).

实验方案

Product Specific Protocols
WB protocol for EDG2 antibody 20442-1-APDownload protocol
IHC protocol for EDG2 antibody 20442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mediators Inflamm

AM966, an Antagonist of Lysophosphatidic Acid Receptor 1, Increases Lung Microvascular Endothelial Permeability through Activation of Rho Signaling Pathway and Phosphorylation of VE-Cadherin.

Authors - Junting Cai
View Article