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验证数据展示

  • WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

    WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 16797-1-AP (same clone as 16797-1-PBS)

    IHC staining of human spleen using 16797-1-AP (same clone as 16797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LITAF antibody included
  • IP experiment of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

    IP experiment of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

    IP result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

    IF Staining of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LITAF Polyclonal antibody, PBS Only

LITAF Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16797-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LITAF Ready-To-Use IHC Kit. LITAF primary antibody included.

产品信息

16797-1-PBS targets LITAF in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10269

Product name: Recombinant human LITAF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC008309

Sequence: MSVPGPYQAATGPSSAPSAPPSYEETVAVNSYYPTPPAPMPGPTTGLVTGPDGKGMNPPSYYTQPAPIPNNNPITVQTVYVQHPITFLDRPIQMCCPSCNKMIVSQLSYNAGALTWLSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipopolysaccharide-induced TNF factor
别名
计算分子量 161 aa, 17 kDa
观测分子量 24 kDa
GenBank蛋白编号BC008309
基因名称 LITAF
Gene ID (NCBI) 9516
RRIDAB_2135710
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99732
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis

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