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验证数据展示

  • WB analysis of HeLa using 16797-1-AP

    WB analysis of HeLa using 16797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 16797-1-AP

    IHC staining of human spleen using 16797-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with LITAF antibody included
  • IP experiment of HeLa using 16797-1-AP

    IP experiment of HeLa using 16797-1-AP

    IP result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug.

  • IF Staining of HepG2 using 16797-1-AP

    IF Staining of HepG2 using 16797-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

LITAF Polyclonal antibody

LITAF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16797-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PIG7, p53-induced gene 7 protein, p53 induced gene 7 protein, LPS-induced TNF-alpha factor, LPS induced TNF alpha factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LITAF Ready-To-Use IHC Kit. LITAF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16797-1-AP targets LITAF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 LITAF fusion protein Ag10269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipopolysaccharide-induced TNF factor
别名 PIG7, p53-induced gene 7 protein, p53 induced gene 7 protein, LPS-induced TNF-alpha factor, LPS induced TNF alpha factor
计算分子量 161 aa, 17 kDa
观测分子量 24 kDa
GenBank蛋白编号BC008309
基因名称 LITAF
Gene ID (NCBI) 9516
RRIDAB_2135710
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99732
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis

实验方案

Product Specific Protocols
WB protocol for LITAF antibody 16797-1-APDownload protocol
IHC protocol for LITAF antibody 16797-1-APDownload protocol
IF protocol for LITAF antibody 16797-1-APDownload protocol
IP protocol for LITAF antibody 16797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Neurobiol

LITAF Enhances Radiosensitivity of Human Glioma Cells via the FoxO1 Pathway.

Authors - Changlin Huang
  • KD Validated
View Article
humanIHC

Nat Commun

Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis

Authors - Shu Zhang
View Article