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CoraLite® Plus 488-conjugated LCK Monoclonal antibody

LCK Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D10C2

Cat No : CL488-60162

Print datasheet

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

验证数据展示

  • IF Staining of HepG2 using CL488-60162

    IF Staining of HepG2 using CL488-60162

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 LCK antibody (CL488-60162, Clone: 2D10C2 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HepG2 using CL488-60162

    FC experiment of HepG2 using CL488-60162

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LCK (CL488-60162, Clone:2D10C2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60162-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60162 targets LCK in IF, FC (Intra) applications and shows reactivity with human, pig, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, pig, rat
Immunogen LCK fusion protein Ag7694 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lymphocyte-specific protein tyrosine kinase
Synonyms LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16
Calculated Molecular Weight 539 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06239
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LCK antibody CL488-60162Download protocol
FC protocol for CL Plus 488 LCK antibody CL488-60162Download protocol
Standard Protocols
Click here to view our Standard Protocols