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LCK Monoclonal antibody

LCK Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

FC, IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Cat No : 60162-1-Ig

Print datasheet

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

验证数据展示

  • WB analysis of pig thymus using 60162-1-Ig

    WB analysis of pig thymus using 60162-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60162-1-Ig

    IHC staining of human tonsillitis using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60162-1-Ig

    IHC staining of human tonsillitis using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60162-1-Ig

    IF Staining of human tonsillitis using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60162-1-Ig

    IF Staining of human tonsillitis using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 60162-1-Ig

    IF Staining of HepG2 using 60162-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 60162-1-Ig

    FC experiment of HepG2 using 60162-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inpig thymus tissue, Ramos cells, rat thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, HepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60162-1-Ig targets LCK in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications FC, IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, pig, rat
Cited Reactivityhuman
Immunogen LCK fusion protein Ag7694 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lymphocyte-specific protein tyrosine kinase
Synonyms LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16
Calculated Molecular Weight 539 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
RRIDAB_10663101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for LCK antibody 60162-1-IgDownload protocol
IHC protocol for LCK antibody 60162-1-IgDownload protocol
IF protocol for LCK antibody 60162-1-IgDownload protocol
FC protocol for LCK antibody 60162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Int J Gen Med

Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis.

Authors - Chao Wang
View Article