验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13708 targets LARP1 in IF/ICC applications and shows reactivity with human, mouse, rat, drosophila samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat, drosophila |
| 免疫原 |
CatNo: Ag4645 Product name: Recombinant human LARP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC033856 Sequence: MPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPTVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHSVVAGGGGGEGRKRCPSQSSSRPAAMISQPPTPPTGQPVREDAKWTSQHSNTQTLGK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | La ribonucleoprotein domain family, member 1 |
| 别名 | KIAA0731, La related protein 1, LARP, LARP1 |
| 计算分子量 | 1096 aa, 124 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC033856 |
| 基因名称 | LARP1 |
| Gene ID (NCBI) | 23367 |
| RRID | AB_3083946 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6PKG0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LARP1, full named as La-related Protein 1, is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. (PMID: 32286153)It's has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). (PMID: 28650797) LARP1 has 2 isoforms with MW of 123-150KDa. With CL647, immunoflorecence detected location in cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LARP1 antibody CL488-13708 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
