验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
For other applications, we recommend the unconjugated version of this antibody, 67324-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67324 targets LAMR1/RPSA in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | LAMR1/RPSA fusion protein Ag6190 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein SA |
| 别名 | LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA |
| 计算分子量 | 33 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC050688 |
| 基因名称 | RPSA |
| Gene ID (NCBI) | 3921 |
| RRID | AB_2934536 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | P08865 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LAMR1/RPSA antibody CL488-67324 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |