验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, U2OS cells, rat lung tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85055-4-RR targets LAMB3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag25192 Product name: Recombinant human LAMB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 828-1172 aa of BC075838 Sequence: AFLMAGQVAEQLRGFNAQLQRTRQMIRAAEESASQIQSSAQRLETQVSASRSQMEEDVRRTRLLIQQVRDFLTDPDTDAATIQEVSEAVLALWLPTDSATVLQKMNEIQAIAARLPNVDLVLSQTKQDIARARRLQAEAEEARSRAHAVEGQVEDVVGNLRQGTVALQEAQDTMQGTSRSLRLIQDRVAEVQQVLRPAEKLVTSMTKQLGDFWTRMEELRHQARQQGAEAVQAQQLAEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVEQIRDHINGRVLYYATCK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | laminin, beta 3 |
| 别名 | Laminin B1k chain, 242442A8, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta |
| 计算分子量 | 1172 aa, 130 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | BC075838 |
| 基因名称 | LAMB3 |
| Gene ID (NCBI) | 3914 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13751 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LAMB3 (β3 subunit of laminin) is an important extracellular matrix protein, which plays an important role in cell adhesion, migration, signal transduction and the formation and stability of basement membrane. LAMB3 plays a key role in the basement membrane of skin and mucosa, maintaining the adhesion between epidermis and dermis. Its abnormal function will lead to skin bullous disease (such as severe borderline epidermolaxis, sJEB), which is characterized by blisters and ulcers on skin and mucosa. LAMB3 is highly expressed in many cancers, including pancreatic ductal adenocarcinoma (PDAC), lung cancer, head and neck squamous cell carcinoma, etc., which is closely related to the proliferation, invasion and metastasis of tumor cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LAMB3 antibody 85055-4-RR | Download protocol |
| IHC protocol for LAMB3 antibody 85055-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |