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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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0
Conjugate
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验证数据展示

  • WB analysis using 85055-4-RR (same clone as 85055-4-PBS)

    WB analysis using 85055-4-RR (same clone as 85055-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85055-4-RR (LAMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85055-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 85055-4-RR (same clone as 85055-4-PBS)

    IHC staining of human colon using 85055-4-RR (same clone as 85055-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85055-4-RR (LAMB3 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85055-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85055-4-RR

    Affinity and Kinetic Characterization of 85055-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85055-4-RR against Human LAMB3 were performed. The affinity constant is 0.244 nM.

    {{ptg:ProductFeaturesInfo}}

LAMB3 Recombinant monoclonal antibody, PBS Only

LAMB3 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85055-4-PBS

产品说明书

CloneNo. 242442A8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

Laminin B1k chain, 242442A8, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85055-4-PBS targets LAMB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag25192

Product name: Recombinant human LAMB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 828-1172 aa of BC075838

Sequence: AFLMAGQVAEQLRGFNAQLQRTRQMIRAAEESASQIQSSAQRLETQVSASRSQMEEDVRRTRLLIQQVRDFLTDPDTDAATIQEVSEAVLALWLPTDSATVLQKMNEIQAIAARLPNVDLVLSQTKQDIARARRLQAEAEEARSRAHAVEGQVEDVVGNLRQGTVALQEAQDTMQGTSRSLRLIQDRVAEVQQVLRPAEKLVTSMTKQLGDFWTRMEELRHQARQQGAEAVQAQQLAEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVEQIRDHINGRVLYYATCK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 laminin, beta 3
别名 Laminin B1k chain, 242442A8, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta
计算分子量 1172 aa, 130 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC075838
基因名称 LAMB3
Gene ID (NCBI) 3914
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13751
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMB3 (β3 subunit of laminin) is an important extracellular matrix protein, which plays an important role in cell adhesion, migration, signal transduction and the formation and stability of basement membrane. LAMB3 plays a key role in the basement membrane of skin and mucosa, maintaining the adhesion between epidermis and dermis. Its abnormal function will lead to skin bullous disease (such as severe borderline epidermolaxis, sJEB), which is characterized by blisters and ulcers on skin and mucosa. LAMB3 is highly expressed in many cancers, including pancreatic ductal adenocarcinoma (PDAC), lung cancer, head and neck squamous cell carcinoma, etc., which is closely related to the proliferation, invasion and metastasis of tumor cells.