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CoraLite® Plus 488-conjugated KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G9C1

Cat No : CL488-66870

Print datasheet

Synonyms

Importin subunit alpha 2, IPOA1, Karyopherin subunit alpha 2, KPNA2, QIP2, RAG cohort protein 1, RCH1, SRP1, SRP1 alpha, SRP1alpha

验证数据展示

  • IF Staining of MCF-7 using CL488-66870

    IF Staining of MCF-7 using CL488-66870

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 KPNA2 antibody (CL488-66870, Clone: 2G9C1 ) at dilution of 1:200.

  • FC experiment of HEK-293T using CL488-66870

    FC experiment of HEK-293T using CL488-66870

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human KPNA2 (CL488-66870, Clone:2G9C1) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inMCF-7 cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy KPNA2 Ready-To-Use IHC Kit. KPNA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66870-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66870 targets KPNA2 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen KPNA2 fusion protein Ag28386 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Synonyms Importin subunit alpha 2, IPOA1, Karyopherin subunit alpha 2, KPNA2, QIP2, RAG cohort protein 1, RCH1, SRP1, SRP1 alpha, SRP1alpha
Calculated Molecular Weight 57.8 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC005978
Gene Symbol KPNA2
Gene ID (NCBI) 3838
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52292
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KPNA2 antibody CL488-66870Download protocol
FC protocol for CL Plus 488 KPNA2 antibody CL488-66870Download protocol
Standard Protocols
Click here to view our Standard Protocols