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验证数据展示

  • IF Staining of LNCaP using CL488-84059-5

    IF Staining of LNCaP using CL488-84059-5

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CoraLite® Plus 488 KLK3/PSA antibody (CL488-84059-5, Clone: 241263F8 ) at dilution of 1:200.

  • FC experiment of PC-3 using CL488-84059-5

    FC experiment of PC-3 using CL488-84059-5

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody (CL488-84059-5, Clone:241263F8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL488-84059-5

    FC experiment of HeLa using CL488-84059-5

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody (CL488-84059-5, Clone:241263F8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody

KLK3/PSA Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84059-5

产品说明书

CloneNo. 241263F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inLNCaP cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84059-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84059-5 targets KLK3/PSA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kallikrein-related peptidase 3
别名 KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77
计算分子量29 kDa
观测分子量 30-34 kDa
GenBank蛋白编号NM_001648.2
基因名称 KLK3
Gene ID (NCBI) 354
RRIDAB_3673335
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07288-1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KLK3/PSA antibody CL488-84059-5Download protocol
Standard Protocols
Click here to view our Standard Protocols