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验证数据展示

  • WB analysis of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    WB analysis of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84059-5-RR (KLK3/PSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 84059-5-RR (same clone as 84059-5-PBS)

    IHC staining of human prostate cancer using 84059-5-RR (same clone as 84059-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84059-5-RR (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSA antibody included
  • IF Staining of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    IF Staining of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using KLK3/PSA antibody (84059-5-RR, Clone: 241263F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84059-5-RR (same clone as 84059-5-PBS)

    FC experiment of HeLa using 84059-5-RR (same clone as 84059-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug KLK3/PSA Recombinant antibody (84059-5-RR, Clone:241263F8) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84059-5-RR

    Affinity and Kinetic Characterization of 84059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84059-5-RR against Human KLK3/PSA were performed. The affinity constant is 149 nM.

KLK3/PSA Recombinant monoclonal antibody, PBS Only

KLK3/PSA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84059-5-PBS

产品说明书

CloneNo. 241263F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

KLK3, 241263F8, APS, EC:3.4.21.77, Gamma-seminoprotein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.

产品信息

84059-5-PBS targets KLK3/PSA in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0869

Product name: Recombinant Human KLK3/PSA protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 18-261 aa of NM_001648.2

Sequence: APLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kallikrein-related peptidase 3
别名 KLK3, 241263F8, APS, EC:3.4.21.77, Gamma-seminoprotein
计算分子量29 kDa
观测分子量 30-34 kDa
GenBank蛋白编号NM_001648.2
基因名称 KLK3
Gene ID (NCBI) 354
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP07288-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

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