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验证数据展示

  • WB analysis of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    WB analysis of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84059-5-RR (KLK3/PSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 84059-5-RR (same clone as 84059-5-PBS)

    IHC staining of human prostate cancer using 84059-5-RR (same clone as 84059-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84059-5-RR (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSA antibody included
  • IF Staining of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    IF Staining of LNCaP using 84059-5-RR (same clone as 84059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using KLK3/PSA antibody (84059-5-RR, Clone: 241263F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84059-5-RR (same clone as 84059-5-PBS)

    FC experiment of HeLa using 84059-5-RR (same clone as 84059-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug KLK3/PSA Recombinant antibody (84059-5-RR, Clone:241263F8) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84059-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84059-5-RR

    Affinity and Kinetic Characterization of 84059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84059-5-RR against Human KLK3/PSA were performed. The affinity constant is 149 nM.

KLK3/PSA Recombinant monoclonal antibody, PBS Only

KLK3/PSA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84059-5-PBS

产品说明书

CloneNo. 241263F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

KLK3, 241263F8, APS, EC:3.4.21.77, Gamma-seminoprotein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.

产品信息

84059-5-PBS targets KLK3/PSA in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0869

Product name: Recombinant Human KLK3/PSA protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 18-261 aa of NM_001648.2

Sequence: APLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kallikrein-related peptidase 3
别名 KLK3, 241263F8, APS, EC:3.4.21.77, Gamma-seminoprotein
计算分子量29 kDa
观测分子量 30-34 kDa
GenBank蛋白编号NM_001648.2
基因名称 KLK3
Gene ID (NCBI) 354
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP07288-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLK3 also known as Prostate-Specific Antigen (PSA), is a 33 kDa glycoprotein primarily synthesized by the epithelial cells of the prostate gland. Its main physiological function is to cleave seminogelins I and II in semen, leading to the liquefaction of the seminal coagulum and the release of motile sperm. It is under tight regulation by androgens via the androgen receptor (AR). Serum PSA levels are elevated in prostate cancer due to disruption of the glandular architecture, allowing more PSA to enter the bloodstream. It is used alongside digital rectal exam (DRE) for early detection. Although termed "prostate-specific," PSA is not exclusively produced by the prostate. Very low levels can be produced in periurethral glands, breast tissue, and some other tissues. Its expression is highly abundant in prostatic tissue and seminal fluid.

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