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KLHL25 Monoclonal antibody

KLHL25 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1D8B8

Cat No : 67774-1-Ig

Print datasheet

Synonyms

ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

验证数据展示

  • WB analysis using 67774-1-Ig

    WB analysis using 67774-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67774-1-Ig

    IHC staining of mouse testis using 67774-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HT-29 cells, HepG2 cells, NCCIT cells, MOLT-4 cells, Raji cells, Ramos cells, Daudi cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67774-1-Ig targets KLHL25 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human, Mouse
Immunogen KLHL25 fusion protein Ag27037 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kelch-like 25 (Drosophila)
Synonyms ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25
Observed Molecular Weight66 kDa
GenBank Accession NumberBC028100
Gene Symbol KLHL25
Gene ID (NCBI) 64410
RRIDAB_2918539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H0H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL25 antibody 67774-1-IgDownload protocol
IHC protocol for KLHL25 antibody 67774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols