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验证数据展示

  • WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

    WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

    IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

    IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

    IF Staining of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14-Specific antibody (14849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KLHL14-Specific Polyclonal antibody, PBS Only

KLHL14-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14849-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14849-1-PBS targets KLHL14-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 14 (Drosophila)
别名 Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14
计算分子量 70 kDa
观测分子量39-45 kDa
GenBank蛋白编号NM_020805
基因名称 KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131171
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2G3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

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