验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse ovary tissue, HepG2 cells, rat brain tissue |
| Positive IHC detected in | human heart tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
14849-1-AP targets KLHL14-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kelch-like 14 (Drosophila) |
| 别名 | Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 39-45 kDa |
| GenBank蛋白编号 | NM_020805 |
| 基因名称 | KLHL14 |
| Gene ID (NCBI) | 57565 |
| RRID | AB_2131171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P2G3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KLHL14-Specific antibody 14849-1-AP | Download protocol |
| IHC protocol for KLHL14-Specific antibody 14849-1-AP | Download protocol |
| WB protocol for KLHL14-Specific antibody 14849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
