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验证数据展示

  • WB analysis of rat brain using 84013-4-RR

    WB analysis of rat brain using 84013-4-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84013-4-RR

    IHC staining of mouse brain using 84013-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84013-4-RR (KIF5A antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using 84013-4-RR

    IF Staining of PC-12 using 84013-4-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of K-562 using 84013-4-RR

    FC experiment of K-562 using 84013-4-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SH-SY5Y using 84013-4-RR

    FC experiment of SH-SY5Y using 84013-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84013-4-RR

    Affinity and Kinetic Characterization of 84013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

KIF5A Recombinant antibody

KIF5A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84013-4-RR

产品说明书

CloneNo. 241124H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, U-87-MG cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inK-562 cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84013-4-RR targets KIF5A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIF5A fusion protein Ag15518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 5A
别名 241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050
计算分子量 1032 aa, 117 kDa
观测分子量 118-120 kDa
GenBank蛋白编号BC146670
基因名称 KIF5A
Gene ID (NCBI) 3798
RRIDAB_3671581
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12840
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

实验方案

Product Specific Protocols
WB protocol for KIF5A antibody 84013-4-RRDownload protocol
IHC protocol for KIF5A antibody 84013-4-RRDownload protocol
IF protocol for KIF5A antibody 84013-4-RRDownload protocol
FC protocol for KIF5A antibody 84013-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols