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验证数据展示

  • WB analysis of rat brain using 84013-4-RR (same clone as 84013-4-PBS)

    WB analysis of rat brain using 84013-4-RR (same clone as 84013-4-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84013-4-RR (same clone as 84013-4-PBS)

    IHC staining of mouse brain using 84013-4-RR (same clone as 84013-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84013-4-RR (KIF5A antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 84013-4-RR (same clone as 84013-4-PBS)

    IF Staining of PC-12 using 84013-4-RR (same clone as 84013-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 84013-4-RR (same clone as 84013-4-PBS)

    FC experiment of K-562 using 84013-4-RR (same clone as 84013-4-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 84013-4-RR (same clone as 84013-4-PBS)

    FC experiment of SH-SY5Y using 84013-4-RR (same clone as 84013-4-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00967-1

    Cytometric bead array standard curve of MP00967-1

    Cytometric bead array standard curve of MP00967-1, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-3-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00967-2

    Cytometric bead array standard curve of MP00967-2

    Cytometric bead array standard curve of MP00967-2, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-1-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84013-4-PBS

    Affinity and Kinetic Characterization of 84013-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 84013-4-PBS against Human KIF5A was performed. The affinity constant is 0.611 nM.

  • Affinity and Kinetic Characterization of 84013-4-RR

    Affinity and Kinetic Characterization of 84013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

KIF5A Recombinant antibody, PBS Only (Capture)

KIF5A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84013-4-PBS

产品说明书

CloneNo. 241124H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84013-4-PBS targets KIF5A as part of a matched antibody pair:

MP00967-1: 84013-4-PBS capture and 84013-3-PBS detection (validated in Cytometric bead array)

MP00967-2: 84013-4-PBS capture and 84013-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIF5A fusion protein Ag15518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 5A
别名 241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050
计算分子量 1032 aa, 117 kDa
观测分子量 118-120 kDa
GenBank蛋白编号BC146670
基因名称 KIF5A
Gene ID (NCBI) 3798
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12840
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.