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验证数据展示

  • WB analysis using CL488-66265

    WB analysis using CL488-66265

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66265 (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using CL488-66265

    IF Staining of mouse brain using CL488-66265

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated KGA-Specific Monoclonal antibody

KGA-Specific Monoclonal Antibody for WB, IF-P
Cat No. CL488-66265

产品说明书

CloneNo. 1B11A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF-P

别名

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse brain tissue
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66265-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66265 targets KGA-Specific in WB, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 KGA-Specific fusion protein Ag24227 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutaminase
别名 AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase
计算分子量 669 aa, 73 kDa
观测分子量 65 kDa
GenBank蛋白编号BC038507
基因名称 GLS
Gene ID (NCBI) 2744
RRIDAB_3084228
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO94925
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 KGA-Specific antibody CL488-66265Download protocol
IF protocol for CL Plus 488 KGA-Specific antibody CL488-66265Download protocol
Standard Protocols
Click here to view our Standard Protocols